In order to calculate a humification index (HIX), spectra were obtained with an excitation wavelength of 254 nm over the emissions waveband of 300–480 nm at increments of 5 nm (Ohno 2002. . Results were plotted as ratio of green/red vs. Amplitude : 1. i3x Multi-Mode Microplate Reader. Keywords: fluorescence polarization, spectramax detection cartridge, spectramax paradigm readers, spectramax paradigm, upgredeable cartridge, Created Date: 8/1/2013 10:01:48. The SpectraTest® Validation Plates are valuable tools for verifying that absorbance-, fluorescence-, and luminescence-capable microplate readers are operating correctly for GMP and GLP laboratories. Time Resolved Fluorescence: Solid white plates for top reads and white plates with clear bottoms for bottom reads. Failed to load PDF file. Description 1. With the SpectraMax® MiniMax™ 300 Imaging Cytometer, industry-leading SoftMax® Pro Software, and user. 在这里,我们展示了如何使用SpectraMax®i3x和 SpectraMax®iD5多功能微孔板读板机应用HTRF技术进行 Gq-偶联受体的分析。G蛋白偶联受体的分析主要通过检测 cAMP和IP3这两条信号转导通路调控介导的细胞内钙离子水 平的升高。Cisbio公司的IP-One Gq试剂盒通过检. It has a cooled PMT, a no-stain cell analysis algorithm, and a field-upgradeable imaging cytometer. SpectraMax Microplate Readers. The SpectraMax® Mini Multi-Mode Microplate Reader offers a budget-friendly solution with three modes of detection for UV-Vis absorbance, fluorescence, and luminescence. applications, including dual luciferase and ATP assays. Facilities. . The SpectraMax MiniMax 300 Imaging Cytometer option is a field-upgradeable option for the SpectraMax i3/i3x Multi-Mode Detection Platform that offers cellular imaging capability. Microplate Readers SpectraMax. QuickSync. The SpectraMax® i3x Multi-Mode Microplate Reader measures absorbance, fluorescence, and luminescence with available upgrades including western blot, cell imaging, and fast kinetics with injectors plus additional detection modes. 1-xiAt last, the relative absorption was estimated using a microplate reader (SpectraMax i3x, Molecular Devices, LLC, Sunnyvale, CA, USA) at 450 nm. SpectraMax i3/i3x Multi-Mode Microplate Reader SoftMax Pro Software 0 500 1000 1500 2000 2500 3000 0 1´ 106 2´ 106 3´ 106 dsDNA (pg/we l) R F U 02 4 6 8 1 0 2. 더 많은. Cell counters for accurate and. 2/5 apart, not needed o Otherwise, ensure cutoff is 10nm less than desired emission wavelength. 030 fmol. Explore Multi-Mode Validation Plate that confirms instrument performance for spectramax i3, i3x, Paradigm system & FilterMax Reader. Data from Analyst®, and FLIPR® systems can also be easily imported and analyzed. 7. Real-time measurement of extracellular oxygen depletion and acidification in cultured cells and microorganisms. 99. 99. . With a preconfigured protocol to simplify data acquisition and analysis, SoftMax Pro Software completes this total solution for fast, sensitive, and reliable dual luciferase reporter assay results. Molecular Interactions. 3. Eliminate time-dependent substrate addition steps. 305 reference level saturation. SeeThe SpectraMax i3x measures monochromator-based absorbance, fluorescence and luminescence with a future-ready platform that can evolve with the researcher’s needs. Description 1. 1321N1, a human astrocytoma cell line. . Note: Additional readers with similar performance are listed. Spectramax I3x, supplied by Danaher Inc, used in various techniques. Spectranet customer portalThe SpectraMax i3x reader and MiniMax cytometer can be used with FluoroBlok cell culture inserts to directly count cells from high-quality images to measure cellular migration over time. The SpectraMax® i3x reader allows you to. Request a Quote for This Setup. All the readers were operated using SoftMax® Pro Software, which also has built-in data analysis and curve-fitting for automatic calculation of EC 50. With optimized reagents and the industry-leading data acquisition and analysis tool SoftMax ® Pro 7 Software, the SpectraMax iD3 allows you to customize your workflow to. The SpectraMax MiniMax 300 Imaging Cytometer option can be added at any time to the SpectraMax i3x System, providing an expandable platform that evolves with your changing needs. Register to download your free eBook today. A SpectraMax® i3x was used to determine fluorescence with an excitation wavelength of λ = 360 nm and emission at λ = 460 nm. each new assay. This eBook shows how to see more and do more with an imaging cytometer that enables rapid imaging and cell analysis. Overview of Western Blot Cartridge. Videos & Webinars. Dual auto injector cartridges for the SpectraMax® i3x Multi-Mode Microplate Detection Platform allows you to expand your research capabilities to include flash based applications, including dual luciferase and ATP assays. Data Analysis. . . The 4x washing steps were also performed on deck using the Span-8 aspirate and dispense for the first three washes. With optimized reagents, option to operate the. The information below applies to a standard 96 well plate, set to a linear shake mode. Enzyme Activation Analysis;. 1. D 1 1. Also refer to this Multimode Reader Comparison Chart. . 1 1 10 100 e e e e e e eSpectraMax Spectrophotometers for UV-Vis Absorbance Detection. 2 nM was calculated using SoftMax Pro Software. The SpectraMax i3x reader provides the. eu@moldev. 314 low lamp level warning. The calculated LOQ values were 0. Reporter Assay. Use the 3. 3: The cuvette chamber. Microplate and read height should be optimized for each new lot of microplates or assay volume used (use the Show Pre-Read Optimization options in the instrument settings in SoftMax Pro Software). Quick imaging and analysis of cells gives you a front-row view of the phenotypic changes that accompany. 0112-0115J 9 Chapter1:Introduction TheSpectraMax®M3Multi-ModeMicroplateReader,SpectraMax®M4Multi-ModeMicroplate Reader,SpectraMax®M5Multi-ModeMicroplateReader,andSpectraMax®M5eMulti-ModeSpectraMax i3x and iD5 readers can be equipped with an HTRF detection cartridge or Enhanced TRF module and filters, respectively. . 2in. 313 reference gain check fail. By using PathCheck, SpectraMax readers can automatically normalize sample absorbance values to a 1-cm pathlength and calculate the concentrations. At each time point, the samples were shaken and fluorescence was measured for SEP (488-nm excitation and 530-nm emission) and. Spectramax I3x, supplied by Danaher Inc, used in various techniques. The SpectraMax i3x Multi-Mode Microplate Reader measures spectral-based absorbance, fluorescence, and luminescence with the added functionality of modular upgrades for western blot, imaging, and fast kinetics with injectors. This document is copyright protected and any reproduction of this document, in whole or any part, is strictly prohibited, except as Molecular Devices may authorize in. Version 7. Spectranet customer portalThe SpectraMax i3x measures monochromator-based absorbance, fluorescence and luminescence with a future-ready platform that can evolve with the researcher’s needs. 0. STAKMAX. Remove the transport bracket and store the retaining screws in the bag that held the 3. Because a PMT needs to be set to a specified gain to reach a specific sensitivity,MDC instruments including: SpectraMax®, VersaMax®, VMax®, EMax®, Gemini, ThermoMax®, and FlexStation®. SpectraDrop Abs Protein Quant (i3-i3x)Certified SpectraMax ® M5 e-HTRF Readers The SpectraMax M5 e reader has the same perfor mance specifications as the M5 but is certified for use with Cisbio Bioassays HTRF (Homogeneous Time-Resolved Fluorescence) technology. With optimized reagents and the industry-leading data acquisition and analysis tool SoftMax® Pro 7 Software, the SpectraMax iD3 allows you to customize your workflow to. User-upgradeable detection modules allow you to expand the capabilities of your microplate reader when your research requires it. . AlphaScreen and AlphaLisa detection using a 680 nm excitation laser diode and a 570/100 nm emission filter. The CellTiter-Glo assay from Promega uses luciferase. Time-resolved fluorescence was measured on the SpectraMax Multi-Mode Microplate Readers (see Table 2 for instrument settings). The intensity of the emitted light can be measured easily with the SpectraMax i3x reader. Description 1. 314 low lamp level warning. Curcumin binding assay. The SpectraMax® i3x Multi-Mode microplate reader measures spectral-based Absorbance, Fluorescence, and Luminescence with the added functionality of modular upgrades for Western Blot, Imaging, and Fast Kinetics with Injectors. ai; ImageXpress Pico; IN Carta Image Analysis Software; CLONE SCREENING. Nucleic Acid and Protein Quantitation. In addition, the iD5 reader measures TRF and FP and can be expanded to include TR-FRET, HTRF®, BRET, dual luciferase reporter assays with injectors, and western blot detection. TR-FRET ratios were calculated by dividing the RFU value for the 665-nm reading by the RFU value for the 616-nm reading, and the resulting numbers were multiplied by 1000. The TUNE detection cartridge uses a breakthrough monochromator design that enables researchers to easily optimize their. Citation 2002). Database contains 1 Molecular Devices SpectraMax i3x Manuals (available for free online viewing or downloading in PDF): Installation manual . Explore more about Optimized settings for Transcreener TR-FRET assay on SpectraMax iD5 and SpectraMax i3x readers. 0´ 105 dsD NA (p g/ wel ) R F UFluorescence intensity was measured using a Spectramax i3x plate reader (Molecular Devices) with excitation at 575 nm (bandwidth 9 nm) and emission at 608 nm (bandwidth 15 nm). SpectraMax i3x and Paradigm readers require the FP-FLUO detection cartridge. Current methods available to rapidly detect these can be resource intensive and thus sub-optimal. 6% for the on-board optics (monochromators). . Italic and boldface type are used for emphasis. We would like to show you a description here but the site won’t allow us. Overview of Western Blot Cartridge. SpectraMax M2 & SpectraMax M2e Multi-mode Plate Readers Operator’s Manual — 0112-0115 Rev. The SpectraMax i3x Multi-mode microplate reader measures spectral-based Absorbance, Fluorescence, and Luminescence with. With optimized reagents and the industry-leading data acquisition and analysis tool SoftMax® Pro 7 Software, the SpectraMax iD3 allows you to customize your workflow to. The SpectraMax® i3 Multi-Mode Detection Platform comes with standard spectral absorbance, fluorescence,. SpectraMax® i3x 多功能读板机配置 SpectraMax® MiniMax™ 300 细胞计数 仪,具有 transmitted light (TL),绿色 (Ex / Em: 460 / 541) 和红色 (Ex / Em: 625 / 713) 荧光通道。MiniMax 细胞计数仪的 SoftMax® Pro 软件可以用来获得各种细 胞的图像并进行分析。本说. 315 can't find zero order. Expand your lab’s capabilities to include flash applications such as dual luciferase and ATP assays with the SpectraMax® i3x injector module with SmartInject® Technology ensuring equal mixing across the plate for high-precision experiments. The SpectraMax i3x reader has variable bandwidth for fluorescence and luminescence, and you are able to choose either 9nm or 15 nm for excitation and either 15 nm or 25 nm for emission. . 5mM EDTA, 1% NP40,Explore a wealth of applications with the future-ready SpectraMax i3x. The SpectraMax i3x reader is engineered to perform with Spectral Fusion Illumination for increased sensitivity across the entire wavelength range, and a cooled photomultiplier tube (PMT) for improved detection in extremely. 50: $75: Improving patient lives by advancing the field of resuscitation medicine. The SpectraMax® i3x Multi-Mode Microplate Reader measures absorbance, fluorescence, and luminescence with available upgrades including western blot, cell imaging, and fast. The SpectraMax i3x is a multi-mode microplate reader that measures absorbance, fluorescence, and luminescence with user-configurable detection modules for western blot, cell imaging, and fast kinetics. The solutions were thoroughly mixed and then incubated in the dark at 20°C for 2 h. The calculated LOQ values were 0. . SpectraMax M3 Plate Read, Plate Map, Data Analysis and Export Setup pg. 과 같은 Parameter를 검사하여 평가할 수 있습니다. Find regional support. **On the SpectraMax iD3/iD5 readers only All graphs are for illustrative purposes only. Danaher Inc spectramax i3x. Using the SpectraMax i3x reader, the preconfigured SpectraMax Glo Steady-Luc Reporter assay protocol in SoftMax Pro Software was used to measure luminescence. The ELISA plates were read at an absorbance of 450 nm on a SpectraMax i3x Plate Reader using Softmax Pro 7. *On the SpectraMax i3x reader only. 1. In luminescence mode, if you are specifying a wavelength for the read, you can select an emission bandwidth of either 15 nm or 25 nm. • Removing the transport locks from the PMT shutters and drawers. SpectraMax and VersaMax Plate Readers Operator’s Manual — 0112-0126 Rev A 5 1. 800. SpectraMax® Microplate Readers and SoftMax® Pro Software. Thetypeof luciferase(Renilla. Additionally, user-installable options allow the SpectraMax i3 System to. Microplate Spectrophotometers. Description: The SpectraMax® iD5 Multi-Mode Microplate Reader is the complete laboratory solution to help you increase your research. SpectraMaxParadigmMulti-ModeDetectionPlatformUserGuide 4 5014038F Chapter5:ReadModesandReadTypes 71 SupportedReadTypes 71 AbsorbanceReadMode 74 FluorescenceIntensityReadMode 79the SpectraMax Paradigm Multi-Mode Detection Platform User Guide. 2 x 10-14. Dilution series of HeLa cells transfected with firefly (red plot) and Renilla (blue plot) luciferases. Quantiferon-TB Gold in Tube Kit. The SpectraMax® i3 Multi-Mode Microplate Reader Detection Platform evolves with your future research needs and offers a breadth of application possibilities. ; The top 3 importers of Spectramax are India with 365. 1、卡盒式模块和细胞成像模块的构架检测功能(如FP、TRF、HTRF、AlphaScreen、ScanLater Western Blot等)卡盒模块化设计,仅通过插入卡盒就能可. SpectraMax® ABS and ABS Plus reader support; ü. . Controls containing all reaction components with either DI water or a fluorescent standard in place of the fluorescent substrate were run alongside the experimental samples. The experiments were performed in triplicates and the spectral reading was recorded using Spectramax i3x microplate reader. Bulk Kit. Certified SpectraMax ® M5 e-HTRF Readers The SpectraMax M5 e reader has the same perfor mance specifications as the M5 but is certified for use with Cisbio Bioassays HTRF (Homogeneous Time-Resolved Fluorescence) technology. Multimode Reader Comparison Chart. This process should take about 3 - 5 minutes. 304 signal level saturation. Spectranet customer portalThe SpectraMax® i3x Multi-Mode microplate reader measures spectral- based Absorbance, Fluorescence, and Luminescence on microplates that range from 6 to 384 wells. Supported Windows Operating System: See release notes. Sensitive TRF detection of two simple, mix-and-measure assay workflows. Specifically, we present neurite. Settings not required by a reader are indicated by ‘---’. *On the SpectraMax i3x reader only. The SpectraMax® 190 spectrophotometer is ideal for most life science applications, especially DNA analysis. Curves were forced through x,y = 0. 1. 蛍光 発光 ELISA. 99 to $1999. Additionally, user-installable options allow the SpectraMax i3 System to grow with your changing application needs to fulfill and go beyond the standard reader applications, protecting your initial investment. The possibility of user upgrading the system with an application cartridge and an imaging cytometer makes this device the most flexible microplate. Capabilities include fluorescence and luminescence readings, environmental control, cell viability assays, enzyme. 304 signal level saturation. FTIR Spectrometer: Shimadzu IRSpirit (2018)The plate was read on the SpectraMax i3x Multi-Mode Microplate Reader. Get more information about Fluorescence Polarization SpectraMax Detection Cartridges, which offer great sensitivity for SpectraMax i3x and Paradigm Systems. Using optimized settings for the Transcreener FP assays presented in this application note, the SpectraMax i3x. The PathCheck Sensor is a revolutionary new awardwinning1 technology in SpectraMax® Instruments for measuring the fluid level in every well of a microplate 2–5. All test measurements and calculations are handledPreconfigured protocols and custom assay workflows simplify microplate data acquisition and analysis. The calculated LOQ values were 0. Explore more about the SpectraMax i3x Multi-Mode Plate Reader, a user-upgradeable detection modules allow you to expand the capabilities of your microplate reader when. 01 0. These robust readers have been placed in labs from Antarctica to the. The SpectraMax DuoLuc Reporter Assay has been performance optimized on the SpectraMax i3x reader with Injector Cartridge. Read height should be optimized for . For more sample throughput, the system canMicroplates were sealed to reduce evaporation (adhesive PCR plate seal; Thermo Fisher Scientific, AB0558) and placed in a microplate reader (Molecular Devices, SpectraMax i3x) for 8 h at 30 °C. Our absorbance plate readers feature our PathCheck Sensor. Order Now. The heating element design of the category 1 microplate readers, such as the SpectraMax i3x Multi-Mode Microplate Reader, applies a dual area sensor for temperature control. Cell viability는 세포막 Integrity 또는. SpectraMax i3x Multi-Mode Detection Platform. . . . Auto PMT Challenge: A standard Photomultiplier Tube (PMT) or detector limits the dynamic range of your instrument. Image analysis provides accurate data. Typically the system can detect absorbance, luminescence, fluorescence, and even make more specialized fluorescence measurements like time-resolved fluorescence (TRF) and fluorescence polarization (FP). Analysis. The SpectraMax® DuoLuc™ Reporter Assay Kit enables highly sensitive quantitation of both firefly and Renilla luciferases in mammalian cells at a great price. com Check our website for a current listing of worldwide distributors. SeeSpectraMax MiniMaxImagingCytometeronpage85. These instruments often feature additional detection modes in addition to fluorescence intensity, such as UV-vis absorbance, fluorescence polarization, and time-resolved. A: percent GFP-positive cells calculated using the classification feature in the software. Video: SpectraMax i3x Overview; Brochure: SpectraMax i3x Multi-Mode Detection PlatformVersaMax, SpectraMax 340PC384, 190, Plus 384 Microplate Readers User Guide Electromagnetic Compatibility Regulatory for Canada (ICES/NMB-001:2006) This ISM device complies with Canadian ICES-001. The SpectraMax® i3x Multi-Mode Microplate Reader measures absorbance, fluorescence, and luminescence with available upgrades including western blot, cell imaging, and fast kinetics with injectors plus additional detection modes. The spectra analysis and K d were determined using Sigmaplot 14. SpectraMax 190: SpectraMax 340PC384: SpectraMax Plus384: VERSAmax: Vmax: Tecan: Sunrise: Trinean: DropSense96: AMBIENT TEMPERATURE STORAGE: Liconic: LPX 220 Hotel: LPX 440 Hotel: Thermo Fisher Scientific: Cytomat 10 Hotel: Cytomat 2 Hotel: Cytomat 24 Hotel: Cytomat 8 Linear Hotel: Cytomat 9 Hotel (MPH) BAR CODE. ü; ü. 2-µL or 4-µL samples were pipetted onto a 64-well SpectraDrop slide in quadruplicate, following the plate map in Figure 2. ATP (“flash” luminescence using . . ImageXpress Confocal HT. Products : SoftMax Pro GxP Software, SoftMax Pro Software. Genscript cPass SARS-CoV-2 Neutralization Antibody Detection. 1 and above: Compatible with only with 64-bit OS. 2 GxP Software is the latest, most secure software to achieve full FDA 21 CFR Part 11 and EudraLex Annex. Microplate optimization should be performed the first time the assay is run, and subsequently for . Negative samples contained only MycoAlert Assay Buffer. 020 fmol 0. Figure 4. #0200-7009) Contact our support team with your questions regarding Molecular Devices products and applications. The SpectraMax i3x Multi-Mode Detection Platform User Guide provides detailed instructions on how to operate, maintain, and troubleshoot the SpectraMax i3x, a versatile and customizable microplate reader that can perform various assays and imaging tasks. i3. Not compatible with Home Edition. Superior flexibility The SpectraMax i3 Multi-Mode Platform comes with standard spectral absorbance, fluorescence, and luminescence detection. Background noise is reduced by the reader’s cooled PMT, enabling increased sensitivity and dynamic range. Fluorescence microplate readers are multimode spectrophotometers with the capability to detect and measure fluorescence emitted by analytes in a multi-well sample format. pathways and protein activation and expression in one system. User-upgradeable detection modules allow you to expand the capabilities of your microp. With the SpectraMax® MiniMax™ 300 Imaging Cytometer, industry-leading SoftMax® Pro Software, and user. The protocols are also available on the SoftMax Pro Software protocol sharing. Price from $9. It can be upgraded with. ; India imports most of its Spectramax from United States, China and Austria and is the largest importer of Spectramax in the World. Detection cartridge modularity allows for easy addition of new read modes within minutes without. Find regional support. The SpectraMax® i3x Multi-Mode Microplate Reader measures absorbance, fluorescence, and luminescence with available upgrades including western blot, cell imaging, and fast. 7 mm for all shake intensities. Located at the right front of the SpectraMax Plus 384, the cuvette chamber has a lid that lifts up, allowing you to insert or remove a cuvette. to View Figures. The chamber. The SpectraMax i3x reader measures spectral-based absorbance, fluorescence, and luminescence with the added functionality of modular upgrades for western blot, imaging, and fast kinetics with injectors. In addition, the iD5 reader measures TRF and FP and can be expanded to include TR-FRET, HTRF®, BRET, dual luciferase reporter assays with injectors, and western blot detection. The SpectraMax Injector System with SmartInject™ Technology features low dead volume (10 pL), overflow protection, and ensures. SpectraMaxi3xMulti-ModeDetectionPlatformUserGuide 72 5039228D. Molecular Devices SpectraMax ® i3x MultiMode Microplate Reader, in combination with luminescent assays for cell viability and cytotoxicity, provides a sensitive and rapid way to measure the number of viable cells in culture and quantify the cytotoxic effects of experimental treatment. Restart the system. After 2 hours of treatment cells were harvested by centrifugation and cell pellets were lysed in RIPA buffer (20mM Tris-Cl, 150mM NaCl, 0. SpectraMax Microplate Readers. The SpectraMax MiniMax 300 Imaging Cytometer option can be added at any time to the SpectraMax i3x System, providing an expandable platform that evolves with your changing needs. )wideand65. ISM Equipment Classification (Group 1, Class A) This equipment is. Bioz Stars score: 98/100, based on 1 PubMed citations. Example: [Setup]. A SpectraMax ® i3x was used to determine uo-rescence with an excitation wavelength of λ = 360 nm and emission at λ = 460 nm. SpectraMax i3x; SpectraMax iD3 / iD5; SpectraMax M Series; SoftMax Pro Software; CELLULAR IMAGING. We would like to show you a description here but the site won’t allow us. VAL014, right table) assays on the SpectraMax i3x reader are shown below. Other technologies such as imaging. NORTH AMERICA +1 800-635-5577 Mon to Fri 7am - 5pm Pacific Time. A SpectraMax® i3x was used to determine fluorescence with an excitation wavelength of λ = 360 nm and emission at λ = 460 nm. StakMax Handling Stacker Base Unit. Explore a wealth of applications with the future-ready SpectraMax i3x. Molecular Devices’ SpectraMax i3x Multi-Mode Microplate Reader and Thermo Fisher Scientific’s Varioskan LUX reader also perform multiplexing. Molecular Devices’ ScanLater Western Blot Detection Cartridge uses TRF detection to turn its SpectraMax i3X and Paradigm plate readers into Western blot readers. Keywords: spectramax m2 systems, spectramax m2e systems, spectramax m2/m2e readers, pathcheck sensor, spectratest abs1 absorbance, stakmax stacker Created Date:. Examples: “Press carefully to engage,” “ Do not. 303 unable to cal dark current. EUROPE +44-118-944-8000 Mon to Fri 8am - 5pm GMT. 306 plate air cal. More Product Information. 6% for the on-board optics (monochromators). This eBook shows how to see more and do more with an imaging cytometer that enables rapid imaging and cell analysis. SpectraMax Quant dsDNA Assay Kits. Rutin was used to generate standard curves for quantification. The SpectraMax i2X is used for cell migration, cell. Title: Fluorescence Intensity SpectraMax Paradigm or i3 Detection Cartridge | Molecular Devices Author: Molecular Devices, LLC Subject: Explore this datasheet to know more about Fluorescence Intensity Detection Cartridges for SpectraMax Paradigm and i3x, which provide speed, sensitivity, and flexibility & uncompromising performance for either top or. Ribonuclease (RNase) R digestion assay For identifying the stabilization of circVRK1, 2 μg isolated RNA from HBMECs was digested by RNase R (3 U/μg; RNR07250, Geneseed, Guangzhou,. **On the SpectraMax iD3/iD5 readers only All graphs are for illustrative purposes only. Figure 1. ZERO BIAS - scores, article reviews, protocol conditions and moreStructured Review. Tailored to your different needs, these assay kits are configured and optimized for Molecular Devices SpectraMax® microplate readers, with preconfigured protocols provided in SoftMax® Pro Software for. ZERO BIAS - scores, article reviews, protocol conditions and moreThe SpectraMax MiniMax 300 Imaging Cytometer option can be added at any time to the SpectraMax i3x System, providing an expandable platform that evolves with your changing needs. 6% for the on-board optics (monochromators). Page 38 SpectraMax iD3 Multi-Mode Microplate Reader User Guide Bottle Holder The bottle holder is mounted on two knobs for ease of installation, cleaning, and replacement. SpectraMax iD5:SpectraMax® iD5 Multi-Mode Microplate Reader - Five-Mode Hybrid Microplate Reader with Automatic NFC Filter Identification and Western Blot Capability, Molecular Devices. The user guide also covers the features and specifications of the SpectraMax MiniMax 300. Average 98 stars, based on 1 article reviews. 2 Parallel Line Analysis Conc log [ng/ML] RLU. Homogeneous and eterogeneous microplate assays can be performed in one flexible system. For each assay kit, a preconfigured protocol is included in SoftMax Pro Software to facilitate the acquisition and analysis of data for a simplified workflow. to View Figures. We would like to show you a description here but the site won’t allow us. The user guide also covers the features, specifications, and software of the reader, as well as the installation and. TP medium was used as a blank and its fluorescence intensity was subtracted from that of mCherry or from chlorophyll auto-fluorescence intensity. This technical note demonstrates validation of the SpectraMax® i3x Multi-Mode Microplate Reader for NanoBRET™ assays. 1 software Download page. Page 28 SpectraMax iD3 Multi-Mode Microplate Reader User Guide 4. SpectraMax iD3 and iD5 Register or Sign In. Additionally, user-installable options allow the SpectraMax i3 System to grow with your changing application needs to fulfill and go beyond the standard reader applications, protecting your initial investment. Here, we demonstrate the benefits of using the SpectraMax ® i3x Multi-Mode Microplate Reader with the SpectraMax ® MiniMax™ 300 Imaging Cytometer in combination with MetaMorph ® Microscopy Automation and Image Analysis Software for performing automated neurite outgrowth imaging and analysis. 58% for the detection module and 2. SpectraMax i3x 多功能微孔板读板机 SpectraMax Paradigm 多功能微孔板读板机 SpectraMax M5e 多功能微孔板读板机 扫一扫关注我 们的官方微信 Molecular Devices 大中华区 全国咨询服务热线: 400-820-3586 Email: info. The SpectraMax i3x microplate reader measures absorbance, luminescence and fluorescence as standard, and provides the flexibility to add novel. 4. 2 GxP Software is the latest, most secure software to achieve full FDA 21 CFR Part 11 and EudraLex Annex 11 compliance with. Tip: MCF-7 cells have a polygonal morphology with irregular dimensions, and they have a habit of clumping into large aggregates. SpectraMaxi3Multi. 303 unable to cal dark current. SpectraMax i3x and Paradigm readers require the FP-FLUO detection cartridge. 100 fmol 1536-well (8 µL) 0. The SpectraMax® i3 Platform from Molecular Devices® is a multi-mode detection system that evolves with your future needs and offers an unlimited breadth of application. Instrument. Supported Windows Operating System: See release notes. 性能特点MD SpectraMax i3X 多功能荧光检测仪为采用光谱融合照明设计、测试功能模块化卡盒设计,具有细胞成像分析单元。. 1. The SpectraMax® i3 Platform from Molecular Devices® is a multi-mode detection system that evolves with your future needs and offers an unlimited breadth of application possibilities. The SpectraMax i3x reader offers a modular design allowing the addition of FP detection to the instrument in just minutes. With the SpectraMax® MiniMax™ 300 Imaging Cytometer, industry-leading SoftMax® Pro Software, and user. Settings not required by a reader are indicated by ‘---’. Determination of anti-inflammatory. The SpectraMax® iD3 Multi-Mode Microplate Reader is the cornerstone of a complete laboratory solution to help you expand the boundaries of your research capabilities. Find our technical support information along with contact details. Eight different automated tests are provided: • Absorbance accuracy (linearity) • Precision (reproducibility) • Stray light • Wavelength accuracy • Wavelength repeatability SoftMax® Pro Software for Windows 10 and 11 is designed to provide the simplicity, flexibility and power required for advanced data analysis. Danaher Inc spectramax i3x Spectramax I3x, supplied by Danaher Inc, used in various techniques. . SpectraMax M2 & SpectraMax M2e Multi-mode Plat e Readers Operator s Manual 0112-0115 Rev. . Biochrom New EX Read 400 Microplate Reader. Concentrating the excitation light on the sample through label specific optics, high levels of sensitivity and performance are reached. The SpectraMax i3x Multi-Mode Detection Platform User Guide provides detailed instructions on how to operate, maintain, and troubleshoot the SpectraMax i3x, a versatile and customizable microplate reader that can perform various assays and imaging tasks. Absorbance Detection Cartridges combine scanning and broad spectrum wavelength range to address a variety of nucleic acids, proteins, ELISAs and immunoassays found in the laboratory. Price from $9. 0´ 104 6. Find regional support. . Sufficient for 1000 reactions. Explore PDF for more details. BRET2canbeusedtostudyvariouskindsofprotein-proteininteractions. Molecular Devices SpectraMax i3x Multi-Mode Detection Platform. Page 9 Setting Up the SpectraMax® MiniMax™ 300 Imaging Cytometer 2. Finally, their fluorescence was measured with 365-nm excitation and 450-nm emission filters using a microplate fluorometer (SpectraMax i3x, Molecular Devices, San Jose, CA, USA) (Saiya-Cork et al. The SpectraMax® i3x offers a wealth of applications; it can measure fluorescence, absorbance and luminescence, and its modular upgrades enable western blotting, imaging cytometry and injectors. 3 STANDARDIZE TO A SINGLE ANALYSIS PLATFORMLearn how you can change the detection module of your SpectraMax i3x instrument in just a few seconds. SpectraMax i3x microplate reader and SoftMax Pro Software protocol. Note: Additional readers with similar performance are listed. See Unpacking the SpectraMax Paradigm Instrument on page 1. Spectranet customer portal. Spectramax i3 manualSpectramax i3 manual Microplate spectramax i3x molecular biocompareSpectramax i3. The SpectraMax Injector Cartridge enables precise reagent delivery and adjustment of delay and integration times that are required to obtain optimal sensitivity for this assay. Microplate reader assays requiring infrared (IR) or red-shifted. . The SpectraMax® Quant™ dsDNA Assay Kits are designed for fluorescence-based dsDNA quantitation across a broad range of concentrations. Antigen Quantitation Protocol. The ScanLater Western Blot Detection Cartridge is a user-installable option for the SpectraMax i3, i3x and Paradigm Multi-Mode Readers. With the SpectraMax® MiniMax™ 300 Imaging Cytometer, industry-leading SoftMax® Pro Software, and user. Unplug the power cable from the back of the instrument.